HTML5 Boilerplate is the professional badass's base HTML/CSS/JS template for a fast, robust and future-proof site.
After more than two years in iterative development, you get the best of the best practices baked in: cross-browser normalization, performance optimizations, even optional features like cross-domain ajax and flash. A starter apache .htaccess config file hooks you the eff up with caching rules and preps your site to serve HTML5 video, use @font-face, and get your gzip zipple on.